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本文摘自【中國(guó)獸醫(yī)學(xué)報(bào)】
益生菌在臨床上應(yīng)用
益生菌在臨床上防治動(dòng)物腹瀉有顯著效果,為探討其作用機(jī)制,將早期斷奶仔豬分為2組,每組10頭,對(duì)照組飼喂基礎(chǔ)日糧,試驗(yàn)組在基礎(chǔ)日糧中添加1‰復(fù)合益生菌,試驗(yàn)期60 d,期間觀(guān)察各實(shí)驗(yàn)動(dòng)物腹瀉情況并記錄腹瀉指數(shù),試驗(yàn)結(jié)束時(shí)每組隨機(jī)選4頭取盲腸食糜及結(jié)腸組織,利用Illumina MiSeq高通量測(cè)序分析盲腸內(nèi)容物微生物結(jié)構(gòu),并用熒光定量PCR方法檢測(cè)結(jié)腸組織TLR/MyD88/NF-κB信號(hào)通路相關(guān)基因轉(zhuǎn)錄水平。結(jié)果顯示,試驗(yàn)組腸道菌群豐度與對(duì)照組比較有很大變化:在門(mén)水平上,試驗(yàn)組擬桿菌門(mén)(Bacteroidetes)平均豐度顯著降低了7.05%(P<0.05),變形菌門(mén)(Proteobacteria)、放線(xiàn)菌門(mén)(Actinobacteria)、厚壁菌門(mén)(Firmicutes)平均豐度提高了4.28%,1.73%,1.50%;在屬水平上,試驗(yàn)組乳桿菌屬(Lactobacillus)的平均豐度顯著降低了18.85%(P<0.05),而瘤胃球菌屬(Ruminococcaceae)的平均豐度顯著提高了8.79%(P<0.05),腸桿菌屬(Enterobacteriaceae)、梭菌屬(Clostridiales)、毛螺菌科(Lachnospiraceae)、鏈球菌屬(Streptococcus)的平均豐度也分別上升了4.02%,3.94%,3.39%,3.01%。同時(shí),試驗(yàn)組IL-10的表達(dá)量顯著高于對(duì)照組(P<0.05),而MyD88、TLR4、NF-κB、IL-6、IL-8、TNF-α表達(dá)量均顯著低于對(duì)照組(P<0.05)。試驗(yàn)組腹瀉指數(shù)較對(duì)照組均有所下降,其中試驗(yàn)15,45 d差異顯著(P<0.05)。可見(jiàn)復(fù)合益生菌增加了腸道微生物的豐度及有益菌的含量,并通過(guò)下調(diào)TLR/MyD88/NF-κB信號(hào)通路的轉(zhuǎn)錄抑制腸道炎癥的發(fā)生,可有效防治動(dòng)物腹瀉。
probiotics have a significant effect on the prevention and treatment of animal diarrhea.In order to explore its mechanism,early weaned piglets were divided into two groups:control group(n=10) and experimental group(n=10).The experimental group was supplemented with 1 ‰ compound probiotics in the basic diet.During the experiment period of 60 days,the diarrhea index of each experimental animal was recorded.At the end of the experiment,4 cecal chyme and colon tissues were randomly selected from each group.Illumina MiSeq high throughput sequencing was used to analyze the microbial structure of cecal content,and fluorescence quantitative PCR was used to detect the transcription level of genes related to TLR/MyD88/NF-κB signaling pathway in colon tissue.The results showed that the abundance of intestinal flora in the experimental group changed greatly compared with the control group.At the gate level,the average abundance of Bacteroidetes in the experimental group decreased by 7.05%(P<0.05).The average abundance of Proteobacteria,Actinobacteria and Firmicutes increased by 4.28%,1.73% and 1.50%,respectively.At the genus level,the average abundance of Lactobacillus was significantly decreased by18.85%,while that of Ruminococcaceae was significantly increased by 8.79%(P<0.05).The average abundance of Enterobacteriaceae,Clostridiales,Lachnospiraceae and Streptococcus also increased by 4.02%,3.94%,3.39%and 3.01%,respectively.At the same time,the expression of IL-10 in the experimental group was significantly higher than that of the control group(P <0.05),while the expression of MyD88,TLR4,NF-κB,IL-6,IL-8 and TNF-αin the experimental group was significantly lower than that of the control group(P<0.05).The diarrhea index of the experimental group was lower than that of the control group,and the difference was significant on the15 th and 45 th day(P<0.05).It can be seen that compound probiotics can increase the abundance of intestinal microorganisms and the content of beneficial bacteria,and inhibit the occurrence of inte
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